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Cytoplasmic Location of DNA Polymerase in Oocytes of the Teleost Fish, Oryzias latipes
http://hdl.handle.net/10424/2962
http://hdl.handle.net/10424/29629fd29951-88ce-48c9-872c-9b765ed65d23
名前 / ファイル | ライセンス | アクション |
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kenshi594350.pdf (296.5 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-04-15 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cytoplasmic Location of DNA Polymerase in Oocytes of the Teleost Fish, Oryzias latipes | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | teleost fish | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA polymerase α | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | oocyte maturation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | isopycnic centrifugation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 硬骨魚 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNAポリメラーゼα | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 卵母細胞成熟 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 等密度遠心 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Iwamatsu, Takashi
× Iwamatsu, Takashi× Haraguchi, Tokuko× Nagano, Hiroshi |
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著者(別言語) | ||||||
岩松, 鷹司 | ||||||
著者(別言語) | ||||||
原口, 徳子 | ||||||
著者(別言語) | ||||||
長野, 弘 | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The intracellular location of DNA polymerase α during oocyte maturation in the teleost fish Oryzias latipes was examined. In order to define the optimum assay conditions, DNA polymerase α from the ovaries of the fish was isolated and partially purified by column chromatography before it was characterized. Optimum conditions for the assay of DNA polymerase α activity were Tris-HCl(pH7.8)as a buffer system and10-20mM Mg2+ as a divalent cation when activated DNA was used as a template primer. In fully grown oocytes, almost all of the DNA polymerase α activity was found in enucleated oocytes but not in isolated germinal vesicles. In addition, analysis by isopycnic centrifugation showed that DNA polymerase α was associated with unknown membranes(probably, vesiculated endoplasmic reticulum)appeared in relatively low density fraction, but not with the endoplasmic reticulum in relatively high density fractions obtained from either immature or mature oocytes. These findings suggest that in oocytes of the fish DNA polymerase α exists in the cytoplasm in a different manner from that of the toad(Nagano et al.,1982). No qualitative change in DNA polymerase α activity was observed during the maturation process of the oocyte. However, the level of DNA polymerase α activity in mature oocytes was approximately2-fold that found in fully grown immature oocytes. | |||||
書誌事項 |
愛知教育大学研究報告. 自然科学編 巻 59, p. 43-50, 発行日 2010-03-01 |
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出版者 | ||||||
出版者 | 愛知教育大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1884-5169 | |||||
書誌情報 | ||||||
愛知教育大学研究報告, 自然科学編. 2010, 59, p. 43-50. | ||||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12466167 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者別名 | ||||||
イワマツ, タカシ | ||||||
著者別名 | ||||||
ハラグチ, トクコ | ||||||
著者別名 | ||||||
ナガノ, ヒロシ | ||||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text |