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Effects of Inhibitors on Forskolin- and Testosterone-Induced Steroid Production by Preovulatory Medaka Follicles
http://hdl.handle.net/10424/252
http://hdl.handle.net/10424/25287807bc1-da3a-4a5d-831a-8b02f516d254
名前 / ファイル | ライセンス | アクション |
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kenshi577379.pdf (296.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2008-10-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effects of Inhibitors on Forskolin- and Testosterone-Induced Steroid Production by Preovulatory Medaka Follicles | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | steroid production | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cyanoketone | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | forskolin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein synthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | medaka follicle | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
Iwamatsu, Takashi
× Iwamatsu, Takashi× Shibata, Yasushi |
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著者(別言語) | ||||||
岩松, 鷹司 | ||||||
著者(別言語) | ||||||
柴田, 安司 | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In follicles of the medaka Oryzias latipes, forskolin(F)stimulated production of both estradiol-17β(E_2) and 17α,20β-dihydroxy-4-pregnen-3-one(17α,20β-diOHP)during 20 hr of incubation, while exogenous testosterone stimulated only the former. Effects of actinomycin D, amanitin, cycloheximide and puromycin on the F and testosterone-induced steroid production were investigated during an in vitro culture system for preovulatory ovarian follicles. When isolated preovulatory follicles were incubated in medium containing actinomycin D, amanitin, cycloheximide or puromycin combined with F or with testosterone, F- and testosterone-induced E_2 and 17α,20β-diOHP production was inhibited. A similar inhibitory effect was also observed with cyanoketone, which is a specific inhibitor of 3β-hydroxy-Δ^5-steroid dehydrogenase(3β-HSD). These results suggest that the F- and testosterone-induced E_2 and 17α,20β-diOHP production in medaka ovarian follicles is probably mediated by de novo synthesis of mRNA. | |||||
書誌事項 |
愛知教育大学研究報告. 自然科学 巻 57, p. 73-79, 発行日 2008-03-01 |
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出版者 | ||||||
出版者 | 愛知教育大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0365-3722 | |||||
書誌情報 | ||||||
愛知教育大学研究報告, 自然科学編. 2008, 57, p. 73-79. | ||||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN0000681X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者別名 | ||||||
イワマツ, タカシ | ||||||
著者別名 | ||||||
シバタ, ヤスシ | ||||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text |