| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-12-11 |
| タイトル |
|
|
タイトル |
Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
chondroitin sulfate E |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bone marrow derived mast cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
sulfotransferase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bafilomycin A |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
GalNAc4S-6ST |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ohtake, Shiori
Kondo, Sachiko
Morisaki, Toshiko
Matsumura, Kaori
Kimata, Koji
Habuchi, Osami
|
| 著者(別言語) |
|
|
著者(別言語) |
羽渕, 脩躬 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bone marrow-derived mast cells (BMMCs) contain chondroitin sulfate (CS)-E comprised of GlcA-GalNAc(4SO_4) units and GlcA-GalNAc(4,6-SO_4) units. GalNAc 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate to position 6 of GalNAc(4SO_4) residues of CS. On the basis of the specificity of GalNAc4S-6ST, it is thought that CS-E is synthesized in BMMC through the sequential sulfation by chondroitin 4-sulfotransferase (C4ST)-1 and GalNAc4S-6ST. In this paper, we investigated whether GalNAc4S-6ST and C4ST-1 are actually expressed in BMMCs in which CS-E is actively synthesized. As the bone marrow cells differentiate to BMMCs, level of C4ST-1 and GalNAc4S-6ST messages increased, whereas chondroitin 6-sulfotransferase (C6ST)-1 message decreased. In the extract of BMMCs, activity of GalNAc4S-6ST and C4ST but not C6ST were detected. The recombinant mouse GalNAc4S-6ST transferred sulfate to both nonreducing terminal and internal GalNAc(4SO_4) residues; the activity toward nonreducing terminal GalNAc(4SO_4) was increased with increasing pH. When CS-E synthesized by BMMCs was metabolically labeled with ^<35>SO_4 in the presence of bafilomycin A, chloroquine or NH_4Cl, the proportion of the nonreducing terminal GalNAc(4,6-SO_4) was increased compared with the control, suggesting that GalNAc4S-6ST in BMMC may elaborate CS-E in the intracellular compartment with relatively low pH where sulfation of the internal GalNAc(4SO_4) by GalNAc4S-6ST preferentially occurs. |
| 書誌事項 |
Biochimica et biophysica acta, General subjects
巻 1780,
号 4,
p. 687-695,
発行日 2008-04
|
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0304-4165 |
| 書誌情報 |
|
|
|
Biochimica et biophysica acta, General subjects. 2008, 1780(4), p. 687-695. |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564679 |
| 権利 |
|
|
権利情報 |
Copyright: 2008 Elsevier B.V. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関係URI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.bbagen.2008.01.004 |
|
|
関連名称 |
http://dx.doi.org/10.1016/j.bbagen.2008.01.004 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1016/j.bbagen.2008.01.004 |
| 著者別名 |
|
|
著者別名 |
ハブチ, オサミ |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
text |
habuchio01.pdf (840.2 kB)
