{"created":"2023-06-20T14:24:11.758028+00:00","id":4835,"links":{},"metadata":{"_buckets":{"deposit":"998569e6-e94e-4c4d-b23f-ec9cace06c9a"},"_deposit":{"created_by":3,"id":"4835","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4835"},"status":"published"},"_oai":{"id":"oai:aue.repo.nii.ac.jp:00004835","sets":["724:726"]},"author_link":["3851","3850","3849","3853","3852","356"],"item_4_biblio_info_7":{"attribute_name":"書誌事項","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"695","bibliographicPageStart":"687","bibliographicVolumeNumber":"1780","bibliographic_titles":[{"bibliographic_title":"Biochimica et biophysica acta, General subjects"}]}]},"item_4_description_27":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bone marrow-derived mast cells (BMMCs) contain chondroitin sulfate (CS)-E comprised of GlcA-GalNAc(4SO_4) units and GlcA-GalNAc(4,6-SO_4) units. GalNAc 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST) transfers sulfate to position 6 of GalNAc(4SO_4) residues of CS. On the basis of the specificity of GalNAc4S-6ST, it is thought that CS-E is synthesized in BMMC through the sequential sulfation by chondroitin 4-sulfotransferase (C4ST)-1 and GalNAc4S-6ST. In this paper, we investigated whether GalNAc4S-6ST and C4ST-1 are actually expressed in BMMCs in which CS-E is actively synthesized. As the bone marrow cells differentiate to BMMCs, level of C4ST-1 and GalNAc4S-6ST messages increased, whereas chondroitin 6-sulfotransferase (C6ST)-1 message decreased. In the extract of BMMCs, activity of GalNAc4S-6ST and C4ST but not C6ST were detected. The recombinant mouse GalNAc4S-6ST transferred sulfate to both nonreducing terminal and internal GalNAc(4SO_4) residues; the activity toward nonreducing terminal GalNAc(4SO_4) was increased with increasing pH. When CS-E synthesized by BMMCs was metabolically labeled with ^<35>SO_4 in the presence of bafilomycin A, chloroquine or NH_4Cl, the proportion of the nonreducing terminal GalNAc(4,6-SO_4) was increased compared with the control, suggesting that GalNAc4S-6ST in BMMC may elaborate CS-E in the intracellular compartment with relatively low pH where sulfation of the internal GalNAc(4SO_4) by GalNAc4S-6ST preferentially occurs.","subitem_description_type":"Abstract"}]},"item_4_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_4_relation_16":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1016/j.bbagen.2008.01.004"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1016/j.bbagen.2008.01.004","subitem_relation_type_select":"DOI"}}]},"item_4_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.bbagen.2008.01.004","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: 2008 Elsevier B.V."}]},"item_4_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564679","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0304-4165","subitem_source_identifier_type":"ISSN"}]},"item_4_text_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"subitem_text_value":"Biochimica et biophysica acta, General subjects. 2008, 1780(4), p. 687-695."}]},"item_4_text_26":{"attribute_name":"著者別名","attribute_value_mlt":[{"subitem_text_value":"ハブチ, オサミ"}]},"item_4_text_4":{"attribute_name":"著者(別言語)","attribute_value_mlt":[{"subitem_text_value":"羽渕, 脩躬"}]},"item_4_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ohtake, Shiori"}],"nameIdentifiers":[{"nameIdentifier":"3849","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kondo, Sachiko"}],"nameIdentifiers":[{"nameIdentifier":"3850","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morisaki, Toshiko"}],"nameIdentifiers":[{"nameIdentifier":"3851","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsumura, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"3852","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimata, Koji"}],"nameIdentifiers":[{"nameIdentifier":"3853","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Habuchi, Osami"}],"nameIdentifiers":[{"nameIdentifier":"356","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90024067","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90024067"},{"nameIdentifier":"90024067","nameIdentifierScheme":"KAKEN - 研究者検索","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000090024067/"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-28"}],"displaytype":"detail","filename":"habuchio01.pdf","filesize":[{"value":"840.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"habuchio01.pdf","url":"https://aue.repo.nii.ac.jp/record/4835/files/habuchio01.pdf"},"version_id":"8d1647f6-2576-45ee-b7df-c522ebbc8065"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chondroitin sulfate E","subitem_subject_scheme":"Other"},{"subitem_subject":"bone marrow derived mast cells","subitem_subject_scheme":"Other"},{"subitem_subject":"sulfotransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"bafilomycin A","subitem_subject_scheme":"Other"},{"subitem_subject":"GalNAc4S-6ST","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["726"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-12-11"},"publish_date":"2009-12-11","publish_status":"0","recid":"4835","relation_version_is_last":true,"title":["Expression of sulfotransferases involved in the biosynthesis of chondroitin sulfate E in the bone marrow derived mast cells"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T16:48:42.372424+00:00"}