{"created":"2023-06-20T14:24:12.239941+00:00","id":4845,"links":{},"metadata":{"_buckets":{"deposit":"e04489d3-4494-482f-9a45-d2679281216d"},"_deposit":{"created_by":3,"id":"4845","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4845"},"status":"published"},"_oai":{"id":"oai:aue.repo.nii.ac.jp:00004845","sets":["724:726"]},"author_link":["3855","119","3899","3900","3894","3898","3896","3897"],"item_4_biblio_info_7":{"attribute_name":"書誌事項","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-07","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"317","bibliographicPageStart":"309","bibliographicVolumeNumber":"230","bibliographic_titles":[{"bibliographic_title":"Planta"}]}]},"item_4_description_27":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_4_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In dicotyledonous plants, nicotianamine synthase (NAS) is thought to play a role in the intercellular transport of iron (Fe). Fe is an essential metal for nitrogen-fixing root nodules of legumes, prompting us to characterize the role of the NAS gene in detail. We previously compared gene-expression profiles in ineffective nodules formed on a Lotus japonicus Fix– mutant, sen1, with those in wild-type effective nodules, and showed that expression of an expressed sequence tag (EST) clone encoding an NAS (EC 2.5.1.43) homologue was repressed in the ineffective nodules. In the present study, two EST clones encoding NAS homologues were found in the EST database. We named them LjNAS1 and LjNAS2. Both were detected as single-copy genes in the L. japonicus genome, and conferred NAS activities in transformed Saccharomyces cerevisiae. LjNAS2 was expressed only in nodules, but LjNAS1 was expressed mainly in leaves, stems, and cotyledons. The level of LjNAS2 transcripts was highest in the nodules 24 days after inoculation with Mesorhizobium loti, and was localized in vascular bundles within the nodules. Expression of LjNAS2 was suppressed in ineffective nodules formed on Fix– mutants other than sen1. By contrast, nitrogenase activities of nodules were not influenced in LjNAS2-suppressed plants. We discuss the role of LjNAS2 from the aspect of Fe translocation in nodules.","subitem_description_type":"Abstract"}]},"item_4_link_3":{"attribute_name":"研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Suganuma, N.","subitem_link_url":"http://souran.aichi-edu.ac.jp/souran/profile.do?id=24BA2I0L&lng=ja"}]},"item_4_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Verlag"}]},"item_4_relation_16":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.springerlink.com/content/12456817uw07571m/"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.springerlink.com/content/12456817uw07571m/","subitem_relation_type_select":"URI"}},{"subitem_relation_name":[{"subitem_relation_name_text":"http://dx.doi.org/10.1007/s00425-009-0944-0"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1007/s00425-009-0944-0","subitem_relation_type_select":"DOI"}}]},"item_4_relation_18":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1007/s00425-009-0944-0","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright: 2009 Springer Verlag"},{"subitem_rights":"http://www.springerlink.com/content/12456817uw07571m/ ; The original publication is avairable at springerlink.com."}]},"item_4_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00775481","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0032-0935","subitem_source_identifier_type":"ISSN"}]},"item_4_text_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"subitem_text_value":"Planta. 2009, 230(2), p. 309-317."}]},"item_4_text_26":{"attribute_name":"著者別名","attribute_value_mlt":[{"subitem_text_value":"スガヌマ, ノリオ"}]},"item_4_text_4":{"attribute_name":"著者(別言語)","attribute_value_mlt":[{"subitem_text_value":"菅沼, 教生"}]},"item_4_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hakoyama, T."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, H."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomita, J."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, A."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, S."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Y."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kouchi, H."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suganuma, N."}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-28"}],"displaytype":"detail","filename":"suganuman002.pdf","filesize":[{"value":"873.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"suganuman002.pdf","url":"https://aue.repo.nii.ac.jp/record/4845/files/suganuman002.pdf"},"version_id":"d7a8c58c-4028-4ee4-9aa9-73c219dfcfca"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fix– mutant","subitem_subject_scheme":"Other"},{"subitem_subject":"Iron","subitem_subject_scheme":"Other"},{"subitem_subject":"Lotus","subitem_subject_scheme":"Other"},{"subitem_subject":"Nicotianamine synthase","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitrogen fixation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["726"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-06"},"publish_date":"2010-07-06","publish_status":"0","recid":"4845","relation_version_is_last":true,"title":["Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T15:12:38.285434+00:00"}