{"created":"2023-06-20T14:20:49.346852+00:00","id":583,"links":{},"metadata":{"_buckets":{"deposit":"75d5c902-9639-4e00-a5ba-822164b9389d"},"_deposit":{"created_by":3,"id":"583","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"583"},"status":"published"},"_oai":{"id":"oai:aue.repo.nii.ac.jp:00000583","sets":["6:74:77"]},"author_link":["127"],"item_3_biblio_info_7":{"attribute_name":"書誌事項","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"58","bibliographicPageStart":"53","bibliographicVolumeNumber":"63","bibliographic_titles":[{"bibliographic_title":"愛知教育大学研究報告. 自然科学編"}]}]},"item_3_description_27":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The polyamine oxidase activity of acetone powder from alfalfa was investigated. No enzyme activity was detected. Oat polyamine oxidase was purified in one step isoelectric point chromatography. This method is described in detail and discussed. The enzyme was homogenously by SDS-PADE. The minimum molecular weight of the enzyme was 51,000 on SDS PAGE. The difference in the Stokes radius of native enzyme and N-glycosidase F-treated enzyme is discussed. The optimal pH of the enzyme was 6.0 for spermidine and spermine. The enzyme activity was reduced by 1 M NaCl to 70.2% and altered the optimal pH from 6.0 to 6.5.","subitem_description_type":"Abstract"}]},"item_3_link_3":{"attribute_name":"研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Sakakibara, Yoko","subitem_link_url":"https://souran.aichi-edu.ac.jp/teachers/56176aa81b8a9770.html"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"愛知教育大学"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12466167","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1884-5169","subitem_source_identifier_type":"ISSN"}]},"item_3_text_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"subitem_text_value":"愛知教育大学研究報告, 自然科学編. 2014, 63, p. 53-58."}]},"item_3_text_26":{"attribute_name":"著者別名","attribute_value_mlt":[{"subitem_text_value":"サカキバラ, ヨウコ"}]},"item_3_text_4":{"attribute_name":"著者(別言語)","attribute_value_mlt":[{"subitem_text_value":"榊原, 洋子"}]},"item_3_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakakibara, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"127","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90242891","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90242891"},{"nameIdentifier":"90242891","nameIdentifierScheme":"KAKEN - 研究者検索","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000090242891/"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-27"}],"displaytype":"detail","filename":"kenshi635358.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kenshi635358.pdf","url":"https://aue.repo.nii.ac.jp/record/583/files/kenshi635358.pdf"},"version_id":"be2fde34-52ea-4483-95c6-8fff9263abbb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"polyamine oxidase","subitem_subject_scheme":"Other"},{"subitem_subject":"alfalfa","subitem_subject_scheme":"Other"},{"subitem_subject":"oat","subitem_subject_scheme":"Other"},{"subitem_subject":"isoelectric point chromatography","subitem_subject_scheme":"Other"},{"subitem_subject":"one step purification","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Evaluation of Alfalfa and Oat Polyamine Oxidase: One-Step Purification and Characteristics","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Evaluation of Alfalfa and Oat Polyamine Oxidase: One-Step Purification and Characteristics","subitem_title_language":"en"}]},"item_type_id":"3","owner":"3","path":["77"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-09"},"publish_date":"2014-04-09","publish_status":"0","recid":"583","relation_version_is_last":true,"title":["Evaluation of Alfalfa and Oat Polyamine Oxidase: One-Step Purification and Characteristics"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T15:58:46.806038+00:00"}