@article{oai:aue.repo.nii.ac.jp:00000594,
 author = {市橋, 正一 and 伊藤, 裕司 and 加藤, 淳太郎},
 journal = {愛知教育大学研究報告. 自然科学編},
 month = {Mar},
 note = {text, Interspecific hybridization is a common method in orchids breeding, because many orchids have highly interspecific crossability. We succeeded to establish interspecific Bletilla hybrids. Although the elite hybrid obtained could propagate vegetatively in vitro, somaclonal variation is a big problem in micropropagation. Seedlings are alternative choice to avoid somaclonal variation but not practical for hybrid cultivars. Establishment of seedling cultivars is expected to provide more advantageous plantlets production. To establish seedling cultivars, use of haploid plant is one of the excellent methods. In seedling varieties, some risks i.e., viral disease, somaclonal mutation, and lower multiplication rate will be reduced. In this study, we investigated to induce haploid and dihaploid plants from female gametes by Naphthalene acetic acid sodium salt (NAA-Na) treatment in diploid Bletilla Briganthes and tetraploid Phalaenopsis, respectively. Unfertilized capsules were produced by treatment of the 2% (w/v) NAA lanoline paste or NAA-Na solution on stigma. Although seeds number harvested was a few, the seeds were cultured on Hyponex medium, containing 3 gL–1 Hyponex (6.5-6-19), 20 gL–1 sucrose and 10 gL–1 agar or on NPCW medium containing 20 gL–1 sucrose and 10 gL–1 agar aseptically. In diploid Ble. Brigantes, some seeds developed into seedlings and the ploidy levels were haploid and diploid (doubled haploid). The seedlings acclimatized successfully were all doubled haploid, raised, and flowered. These NAA-Na induced plants produced fertile seeds of both self and cross fertilization. In tetraploid Phalaenopsis, the ploidy levels of seedlings which germinated from NAA-Na induced seeds were dihaploid only. These seedling showed poor growth and no acclimatized seedlings was obtained. These results suggest that seedling F1 cultivars might be realistic in Ble. Brigantes. To establish seedling F1 cultivar in Phalaenopsis, much investigation is required.},
 pages = {25--33},
 title = {シランの品種育成とF1品種化を目指したラン科植物のナフタレン酢酸ナトリュウム塩柱頭処理による還元性雌性配偶子からの半数体と二ゲノム性半数体の誘導},
 volume = {62},
 year = {2013}
}