{"created":"2023-06-20T14:20:51.072592+00:00","id":608,"links":{},"metadata":{"_buckets":{"deposit":"42dea6e5-bd63-450d-b059-7cbc22d0e6e0"},"_deposit":{"created_by":3,"id":"608","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"608"},"status":"published"},"_oai":{"id":"oai:aue.repo.nii.ac.jp:00000608","sets":["6:74:79"]},"author_link":["858","136"],"item_3_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Multiplication of Dactylorhiza aristata Fisch. (Orchidaceae) through Aseptic Seed Culture"}]},"item_3_biblio_info_7":{"attribute_name":"書誌事項","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"29","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"愛知教育大学研究報告. 自然科学編"}]}]},"item_3_description_27":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Culture conditions and culture media of aseptic seed culture were investigated to establish the multiplication of Dactylorhiza aristata Fisch. (Orchidaceae). Immature seeds, which are 3 weeks old after pollination, could germinate and 5 weeks old seeds showed the best germination. Chilling increased germination rate of mature seeds on Hyponex media. Addition of 1 ppm BA and/or 1 ppm NAA to Hyponex media showed no effect on germination. Germination on Norstog media showed higher germination rate than that on Hyponex media. Chilling did not stimulate seed germination on Norstog medium but stimulated seed germination on Hyponex media. Addition of NH_4NO_3 into Norstog medium inhibited germination and the cause of inhibition was NO_3–. Inhibitory effect of NO_3– was cancelled by chilling and no chilling effect was observed on Norstog medium which contained no NO_3–. Growth of protocorms was inhibited lesser extent by NO_3– than germination of seed. Growth of protocorms was stimulated by addition of peptone or potato extract to culture media.","subitem_description_type":"Abstract"},{"subitem_description":"ハクサンチドリの無菌発芽法による増殖法を検討した。ハイポネックス培地に播種したハクサンチドリ未熟種子は、授粉後3週目で発芽が認められ、授粉後5週目で発芽数は最高になった。 ハイポネックス培地に播種したハクサンチドリ完熟種子の発芽には、低温処理の効果が認められた。 BA（1 ppm）とNAA（1 ppm）添加の発芽に対する効果はみられなかった。 Hyponex培地と比較して、Norstog培地での発芽が勝り、同培地では低温処理の効果は認められなかった。 Norstog培地にNH_4NO_3を添加すると、ハクサンチドリの発芽は有意に抑制され、その原因は硝酸態窒素の発芽抑制効果によるものであった。 硝酸態窒素を含む培地での発芽には、低温処理が有効で、無機態窒素を含まないNorstog培地での発芽には低温処理の効果はなかった。 ハクサンチドリのプロトコームの生育においても硝酸態窒素の阻害的効果が示唆されたが、種子発芽の場合よりも影響は少なかった。 ハクサンチドリのプロトコームの生育にはペプトン添加およびジャガイモ抽出物添加の効果が認められた。","subitem_description_type":"Abstract"}]},"item_3_link_3":{"attribute_name":"研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"市橋, 正一","subitem_link_url":"https://souran.aichi-edu.ac.jp/person/37693cfc748049e45d87b8c7d8b9aacdja.html"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"愛知教育大学"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12466167","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1884-5169","subitem_source_identifier_type":"ISSN"}]},"item_3_text_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"subitem_text_value":"愛知教育大学研究報告, 自然科学編. 2012, 61, p. 29-37."}]},"item_3_text_26":{"attribute_name":"著者別名","attribute_value_mlt":[{"subitem_text_value":"イチハシ, ショウイチ"},{"subitem_text_value":"イチカワ, ヤスコ"}]},"item_3_text_4":{"attribute_name":"著者(別言語)","attribute_value_mlt":[{"subitem_text_value":"Ichihashi, Syoichi"},{"subitem_text_value":"Ichikawa, Yasuko"}]},"item_3_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"市橋, 正一"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"市川, 泰子"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-27"}],"displaytype":"detail","filename":"kenshi612937.pdf","filesize":[{"value":"4.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kenshi612937.pdf","url":"https://aue.repo.nii.ac.jp/record/608/files/kenshi612937.pdf"},"version_id":"a24c9820-6d21-4db0-8430-7435305c4625"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ハクサンチドリ","subitem_subject_scheme":"Other"},{"subitem_subject":"Dactylorhiza aristata","subitem_subject_scheme":"Other"},{"subitem_subject":"発芽","subitem_subject_scheme":"Other"},{"subitem_subject":"Germination","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ハクサンチドリの無菌発芽法による増殖","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ハクサンチドリの無菌発芽法による増殖"}]},"item_type_id":"3","owner":"3","path":["79"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-03-29"},"publish_date":"2012-03-29","publish_status":"0","recid":"608","relation_version_is_last":true,"title":["ハクサンチドリの無菌発芽法による増殖"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T15:31:33.750268+00:00"}