{"created":"2023-06-20T14:20:55.182463+00:00","id":678,"links":{},"metadata":{"_buckets":{"deposit":"4240f2bd-f23a-4cdd-9f76-5eb25d81c5e7"},"_deposit":{"created_by":3,"id":"678","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"678"},"status":"published"},"_oai":{"id":"oai:aue.repo.nii.ac.jp:00000678","sets":["6:74:84"]},"author_link":["127","874"],"item_3_biblio_info_7":{"attribute_name":"書誌事項","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"49","bibliographicPageStart":"45","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"愛知教育大学研究報告. 自然科学"}]}]},"item_3_description_27":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The common protein streptavidin, and the n-type glycoproteins ovalbumin and transferrin were eluted at their respective isoelectric points from CM-Sephadex, CM-Sepharose, SP-Sephadex, and SP-Sepharose by０．１M Tris succinate buffer. No tailing occurred with a linear gradient where the ionic strength was gradually decreased. Although streptavidin and a large proportion of ovalbumin were not absorbed by CM-Sephadex equilibrated with a０．２M buffer, transferrin and some of the ovalbumin were eluted at their isoelectric points. This method should be called isoelectric point column chromatography because compounds are eluted from the column at their isoelectric points. This method may be suitable for use with various proteins. In particular, glycoproteins, which are considered to be difficult to purify, can be easily eluted at their isoelectric points under high ionic strength.","subitem_description_type":"Abstract"}]},"item_3_link_3":{"attribute_name":"研究者総覧へのリンク","attribute_value_mlt":[{"subitem_link_text":"Sakakibara, Yoko","subitem_link_url":"https://souran.aichi-edu.ac.jp/teachers/56176aa81b8a9770.html"}]},"item_3_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"愛知教育大学"}]},"item_3_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0000681X","subitem_source_identifier_type":"NCID"}]},"item_3_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0365-3722","subitem_source_identifier_type":"ISSN"}]},"item_3_text_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"subitem_text_value":"愛知教育大学研究報告, 自然科学編. 2007, 56, p. 45-49."}]},"item_3_text_26":{"attribute_name":"著者別名","attribute_value_mlt":[{"subitem_text_value":"サカキバラ, ヨウコ"},{"subitem_text_value":"ヤナギサワ, ヒロシ"}]},"item_3_text_4":{"attribute_name":"著者(別言語)","attribute_value_mlt":[{"subitem_text_value":"榊原, 洋子"},{"subitem_text_value":"柳澤, 寛"}]},"item_3_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakakibara, Yoko"}],"nameIdentifiers":[{"nameIdentifier":"127","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90242891","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90242891"},{"nameIdentifier":"90242891","nameIdentifierScheme":"KAKEN - 研究者検索","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000090242891/"}]},{"creatorNames":[{"creatorName":"Yanagisawa, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"874","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-27"}],"displaytype":"detail","filename":"kenshi564549.pdf","filesize":[{"value":"292.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kenshi564549.pdf","url":"https://aue.repo.nii.ac.jp/record/678/files/kenshi564549.pdf"},"version_id":"8c7bf57a-6902-42e7-9a3d-469ac0e65fae"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Isoelectric point","subitem_subject_scheme":"Other"},{"subitem_subject":"Chromatography","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycoprotein","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Ionic strength","subitem_subject_scheme":"Other"},{"subitem_subject":"Separation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Techniques for the separation of proteins by isoelectric point column chromatography","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Techniques for the separation of proteins by isoelectric point column chromatography","subitem_title_language":"en"}]},"item_type_id":"3","owner":"3","path":["84"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-10-24"},"publish_date":"2008-10-24","publish_status":"0","recid":"678","relation_version_is_last":true,"title":["Techniques for the separation of proteins by isoelectric point column chromatography"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T15:58:43.930417+00:00"}