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The Distribution of Actin Filaments in the Egg of the Rose Bitterling during the Sperm Entry Process in Monospermic Fertilization
http://hdl.handle.net/10424/1722
http://hdl.handle.net/10424/1722ea30e2a6-206c-48af-88b3-525354aa05ac
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kenshi583541.pdf (396.7 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-05-07 | |||||
| タイトル | ||||||
| タイトル | The Distribution of Actin Filaments in the Egg of the Rose Bitterling during the Sperm Entry Process in Monospermic Fertilization | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fish egg | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | actin filament | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fertilization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | rhodamine phalloidin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fluorescence microscopy | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Ohta, Tadayuki
× Ohta, Tadayuki× Yamada, Masato× Hirota, Satoshi |
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| 著者(別言語) | ||||||
| 太田, 忠之 | ||||||
| 著者(別言語) | ||||||
| 山田, 雅人 | ||||||
| 著者(別言語) | ||||||
| 廣田, 智史 | ||||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The distribution of actin filaments and morphological changes in the portion of egg cortex（PAS）at and around the sperm entry site during sperm entry in eggs of a teleost undergoing monospermic fertilization were investigated by using fluorescence and scanning electron microscopy. The fish studied was the rose bitterling, Rhodeus ocellatus ocellatus. The fertilized spermatozoon moves from the sperm entry site to the inner cortex to fuse with the egg nucleus. Actin filaments have been suggested as possible candidates for cell organelles that take part in the movement of the sperm head based on results obtained in studies using the actin polymerization inhibitor, cytochalasin B（CB, Ohta et al.,1998）. In the present study, the validity of this suggestion was further examined by using rhodamine phalloidin（RhPh）, which specifically stained actin filaments. The PAS, which is devoid of cortical alveoli and adheres to the inside of the egg membrane（chorion）, was stained strongly with RhPh in eggs fixed from one to fifteen min after insemination. On the other hand, the PAS was only faintly stained with RhPh in CB-treated eggs. This report presents evidence that a number of actin filaments are present in the PAS which separates from the rest of the egg after sperm penetration. | |||||
| 書誌事項 |
愛知教育大学研究報告, 自然科学編 巻 58, p. 35-41, 発行日 2009-03-01 |
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| 出版者 | ||||||
| 出版者 | 愛知教育大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0365-3722 | |||||
| 書誌情報 | ||||||
| 愛知教育大学研究報告, 自然科学編. 2009, 58, p. 35-41. | ||||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0000681X | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者別名 | ||||||
| オオタ, タダユキ | ||||||
| 著者別名 | ||||||
| ヤマダ, マサト | ||||||
| 著者別名 | ||||||
| ヒロタ, サトシ | ||||||
| 著者別名 | ||||||
| Ota, Tadayuki | ||||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
