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Techniques for the separation of proteins by isoelectric point column chromatography
http://hdl.handle.net/10424/201
http://hdl.handle.net/10424/20197f2bd3b-cd7e-4e93-8754-63fcb7296dab
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kenshi564549.pdf (292.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-24 | |||||
| タイトル | ||||||
| タイトル | Techniques for the separation of proteins by isoelectric point column chromatography | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Isoelectric point | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chromatography | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Glycoprotein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ionic strength | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Separation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Sakakibara, Yoko
× Sakakibara, Yoko× Yanagisawa, Hiroshi |
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| 研究者総覧へのリンク | ||||||
| 表示名 | Sakakibara, Yoko | |||||
| URL | https://souran.aichi-edu.ac.jp/teachers/56176aa81b8a9770.html | |||||
| 著者(別言語) | ||||||
| 値 | 榊原, 洋子 | |||||
| 著者(別言語) | ||||||
| 値 | 柳澤, 寛 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The common protein streptavidin, and the n-type glycoproteins ovalbumin and transferrin were eluted at their respective isoelectric points from CM-Sephadex, CM-Sepharose, SP-Sephadex, and SP-Sepharose by０．１M Tris succinate buffer. No tailing occurred with a linear gradient where the ionic strength was gradually decreased. Although streptavidin and a large proportion of ovalbumin were not absorbed by CM-Sephadex equilibrated with a０．２M buffer, transferrin and some of the ovalbumin were eluted at their isoelectric points. This method should be called isoelectric point column chromatography because compounds are eluted from the column at their isoelectric points. This method may be suitable for use with various proteins. In particular, glycoproteins, which are considered to be difficult to purify, can be easily eluted at their isoelectric points under high ionic strength. | |||||
| 書誌事項 |
愛知教育大学研究報告. 自然科学 巻 56, p. 45-49, 発行日 2007-03-01 |
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| 出版者 | ||||||
| 出版者 | 愛知教育大学 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0365-3722 | |||||
| 書誌情報 | ||||||
| 値 | 愛知教育大学研究報告, 自然科学編. 2007, 56, p. 45-49. | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0000681X | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者別名 | ||||||
| 値 | サカキバラ, ヨウコ | |||||
| 著者別名 | ||||||
| 値 | ヤナギサワ, ヒロシ | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
