| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2010-07-06 |
| タイトル |
|
|
タイトル |
Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Fix– mutant |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Iron |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Lotus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nicotianamine synthase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Nitrogen fixation |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hakoyama, T.
Watanabe, H.
Tomita, J.
Yamamoto, A.
Sato, S.
Mori, Y.
Kouchi, H.
Suganuma, N.
|
| 研究者総覧へのリンク |
|
|
|
Suganuma, N. |
|
|
http://souran.aichi-edu.ac.jp/souran/profile.do?id=24BA2I0L&lng=ja |
| 著者(別言語) |
|
|
著者(別言語) |
菅沼, 教生 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In dicotyledonous plants, nicotianamine synthase (NAS) is thought to play a role in the intercellular transport of iron (Fe). Fe is an essential metal for nitrogen-fixing root nodules of legumes, prompting us to characterize the role of the NAS gene in detail. We previously compared gene-expression profiles in ineffective nodules formed on a Lotus japonicus Fix– mutant, sen1, with those in wild-type effective nodules, and showed that expression of an expressed sequence tag (EST) clone encoding an NAS (EC 2.5.1.43) homologue was repressed in the ineffective nodules. In the present study, two EST clones encoding NAS homologues were found in the EST database. We named them LjNAS1 and LjNAS2. Both were detected as single-copy genes in the L. japonicus genome, and conferred NAS activities in transformed Saccharomyces cerevisiae. LjNAS2 was expressed only in nodules, but LjNAS1 was expressed mainly in leaves, stems, and cotyledons. The level of LjNAS2 transcripts was highest in the nodules 24 days after inoculation with Mesorhizobium loti, and was localized in vascular bundles within the nodules. Expression of LjNAS2 was suppressed in ineffective nodules formed on Fix– mutants other than sen1. By contrast, nitrogenase activities of nodules were not influenced in LjNAS2-suppressed plants. We discuss the role of LjNAS2 from the aspect of Fe translocation in nodules. |
| 書誌事項 |
Planta
巻 230,
号 2,
p. 309-317,
発行日 2009-07
|
| 出版者 |
|
|
出版者 |
Springer Verlag |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0032-0935 |
| 書誌情報 |
|
|
|
Planta. 2009, 230(2), p. 309-317. |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00775481 |
| 権利 |
|
|
権利情報 |
Copyright: 2009 Springer Verlag |
| 権利 |
|
|
権利情報 |
http://www.springerlink.com/content/12456817uw07571m/ ; The original publication is avairable at springerlink.com. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関係URI |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://www.springerlink.com/content/12456817uw07571m/ |
|
|
関連名称 |
http://www.springerlink.com/content/12456817uw07571m/ |
| 関係URI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s00425-009-0944-0 |
|
|
関連名称 |
http://dx.doi.org/10.1007/s00425-009-0944-0 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
info:doi/10.1007/s00425-009-0944-0 |
| 著者別名 |
|
|
著者別名 |
スガヌマ, ノリオ |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
text |
suganuman002.pdf (873.0 kB)
